The Spleen channel originates at Yinbai (SP 1). It runs along the medial aspect of the foot at the junction of the red and white skin and ascends in front of the medial malleolus up to the medial aspect of the leg. It follows the posterior aspect of the tibia, crossing in front of the Liver channel. Passing through the anterior medial aspect of the knee and thigh, it crosses Zhongji (CV 3) and Guanyuan (CV 4) and enters the abdomen. Then passes through Xiawan (CV 10) to the SP organ and connects with the ST. From there it ascends, passing through the diaphragm at Riyue (GB 24), Qimen (LR 14), and Zhongfu (LU 1), and runs alongside the esophagus. When it reaches the root of the tongue, it spreads over its lower surface.
The branch from the ST goes upward through the diaphragm, and enters the HT, where it connect with the HT channel.